Check Initial Scan Parameters

Scan Panel

In the Scan panel, set the following initial scan parameters:

• Scan Rate: 0.5 Hz
• Scan Size: 500 nm
• Scan Angle: 0

Feedback Panel

For Contact Mode:

• Deflection Setpoint: 2 V more than the vertical deflection during laser alignment
• Integral Gain: 5
• Proportional Gain: 10

For TappingMode:

• Integral Gain: 0.5
• Proportional Gain: 5
• It is not necessary to enter a value for Amplitude Setpoint for TappingMode because this value is automatically determined during the engage process.

Channels 1 and 2

• Channel 1 Data Type: Height Sensor
• Channel 2 Data Type: Deflection Error in Contact Mode or Amplitude Error in TappingMode
• Data Scale: choose a reasonable value for the sample. For example, for a 200 nm step height calibration sample, a reasonable initial Data Scale setting is 300 nm. The Data Scales will automatically be adjusted after a few seconds of scanning by the Autoscale function.
• Line Direction: Trace or Retrace
• Channel 3 through Channel 8 Data Type: Off

Previous Steps:

1. Fluid Set Up
2. Align the Laser
3. Load the Sample and Adjust the SPM Scanner
4. Locate the Tip
5. Tune the Cantilever (TappingMode Only)
6. Focus on the Sample Surface

Next Steps:

8. Engage and Adjust Scan Parameters
9. Withdraw and Clean Up

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